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Kröger Group - Publications


Natural hybrid silica/protein superstructure at atomic resolution

S. Görlich, A. J. Samuel, R. J. Best, R. Seidel, J. Vacelet, F. K. Leonarski, T. Tomizaki, B. Rellinghaus, D. Pohl, I. Zlotnikov

PNAS (2020)

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta

G. Bilcke, K. Van den Berge, S. De Decker, E. Bonneure, N. Poulsen, P. Bulankova, C. M. Osuna-Cruz, J. Dickenson, K. Sabbe, G. Pohnert, K. Vandepoele, S. Mangelinckx, L. Clement, L. De Veylder, W. Vyverman

ISME Journal (2020)

An intimate view into the silica deposition vesicles of diatoms

C. Heintze, P.Formanek, D. Pohl, J. Hauptstein, B. Rellinghaus, N.Kröger

BMC Materials 2:11 (2020)

Chitin synthase localization in the diatom Thalassiosira pseudonana

M. Wustman, N. Poulsen, N. Kröger, K.H. van Pée

BMC Materials 2: 10 (2020)

The Seminavis robusta genome provides insights into the evolutionary adaptations of benthic diatoms

C. Osuna-Cruz et al.

Nature Communications, 11, Article: 3320

Genetically Programmed Regioselective Immobilization of Enzymes in Biosilica Microparticles

E. Kumari, S. Görlich, N. Poulsen, N. Kröger

Adv. Funct. Mater., 30, 2000442 (2020)


Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis

M. Lachnit, M.T. Buhmann, J. Klemm, N. Kröger, N. Poulsen*

Phil. Trans. R. Soc. B., 374: 20190196 (2019)

Control of biosilica morphology and mechanical performance by the conserved diatom gene Silicanin-1

S. Görlich, D. Pawolski, I. Zlotnikov, N. Kröger

Commun. Biol. 2, 245 (2019)

Influence of silica architecture on the catalytic activity of immobilized glucose oxidase

G. Begum, C. Oschatz, M. Oschatz, S. Kaskel, E. Brunner, N. Kröger

Bioinspired Biomimetic and Nanobiomaterials 8, 72-80 (2019)


Biomineralization as a paradigm of directional solidification: A physical model for molluscan shell ultrastructural morphogenesis

V. Schoeppler, L. Gránásy, E. Reich, N. Poulsen, R. de Kloe, P. Cook, A. Rack, T. Pusztai, I. Zlotnikov*

Adv. Mater. 30 , 1803855 (2018)

Reconstituting the formation of hierarchically porous silica patterns using diatom biomolecules

D. Pawolski, C. Heintze, I. Mey, C. Steinem, N. Kröger

J. Struct. Biol. 204, 64-74 (2018)


Immobilization of Proteins on Diatom Biosilica

N. Kröger, N. C. Dubey, E. Kumari

Diatom Nanotechnology (D. Losic, ed.), RSC Publishing p. 126-149 (2017)

Silicanin-1 is a conserved diatom membrane protein involved in silica biomineralization

A. Kotzsch, P. Gröger, D. Pawolski, P. H. H. Bomans, N. A. J. M. Sommerdijk, M. Schlierf, N. Kröger

BMC Biol. 15, 65 (2017)


Establishing super-resolution imaging for proteins in diatom biosilica

P. Gröger, N. Poulsen, J. Klemm, N. Kröger, M. Schlierf

Scientific Reports 6, Article number: 36824 (2016)

Structures of the PSCD domains of pleuralin-1, a biosilica-associated protein from the diatom Cylindrotheca fusiformis, and their interactions with other cell wall proteins

S. De Sanctis, M. Wenzler, N. Kröger, W. M. Malloni, M. Sumper, R. Deutzmann, P. Zadravec, E. Brunner, W. Kremer, H. R. Kalbitzer

Structure 24, 1178–1191 (2016)

Biochemical composition and assembly of biosilica-associated insoluble organic matrices from the diatom Thalassiosira pseudonana

A. Kotzsch, D. Pawolski, A. Milentyev, Anna Shevchenko, A. Scheffel, N. Poulsen, Andrej Shevchenko, N. Kröger

J. Biol. Chem. 291, 4982-4997 (2016)


Targeted drug delivery using genetically engineered diatom biosilica

B. Delalat, V. Sheppard, S. Rasi Ghaemi, S. Rao, C. Prestidge, G. McPhee, M.-L. Rogers, J. Donoghue, V. Pillay, T. Johns, N. Kröger*, N. Voelcker*

Nat. Commun. 6, 8791 (2015)

Compartmentalisation of Enzymes for Cascade Reactions through Biomimetic Layer-by-Layer Mineralization

G. Begum, W. B. Goodwin, B. M. deGlee, K. H. Sandhage, N. Kröger

J. Mater. Chem. B, 3, 5232-5240 (2015)


A Tyrosine-rich Cell Surface Protein in the Diatom Amphora coffeaeformis identified through Transcriptome Analysis and Genetic Transformation

M. T. Buhmann, N. Poulsen, J. Klemm, M. R. Kennedy, C. D. Sherrill, N. Kröger

PLOS ONE 9, e110369 (2014)

Complex-shaped microbial biominerals for nanotechnology

N. Kröger, E. Brunner

WIREs Nanomed. Nanobiotechnol. 6, 615-627 (2014)

Isolation and Biochemical Characterization of Underwater Adhesives from Diatoms

N. Poulsen*, N. Kröger , M. J. Harrington, E. Brunner, S. Paasch and M. T. Buhmann

Biofouling 30, 513-523 (2014)


Nitrate and carbonate concentration for high glucose content in microalgae

N. Kröger, S. J. Miller, N. Poulsen

US patent US8569040 B1 (approved)

Pentalysine clusters mediate silica targeting of silaffins in Thalassiosira pseudonana

N. Poulsen, A. Scheffel, V. C. Sheppard, P. M. Chesley, N. Kröger

J. Biol Chem. 288, 20100-20109 (2013)

Kieselalgen für die Nanotechnik

N. Kröger

Nachrichten aus der Chemie 61, 514-519 (2013)

Rapid Flow-through Biocatalysis with High Surface Area, Enzyme -loaded Carbon and Gold- bearing Diatom Frustule Replicas

S. C. Davis, V. C. Sheppard, G. Begum, Y. Cai, Y. Fang, J. D. Berrigan, N. Kröger,* Kenneth H. Sandhage*

Adv. Funct. Mater. 23, 4611-4620 (2013)

Morphogenese der Silica-Zellwände von Diatomeen

N. Kröger

BIOspektrum 19, 12-15 (2013)


Live Diatom Silica Immobilization of Multimeric and Redox-active Enzymes

V. C. Sheppard, A. Scheffel, N. Poulsen, N. Kröger

Appl. Environ. Microbiol. 78, 211-218 (2012).


Biocatalytic Nanoscale Coatings through Biomimetic Layer-by-Layer Mineralization

N. R. Haase, S. Shian, K. H. Sandhage, N. Kröger

Adv. Funct. Mater. 21, 4243-4251 (2011).

Nanopatterned protein microrings from a diatom that direct silica morphogenesis

A. Scheffel, N. Poulsen, S. Shian, N. Kröger

Proc. Natl. Acad. Sci. USA 108, 3175-3180 (2011).


From Diatom Biomolecules to Bioinspired Syntheses of Silica- and Titania-based Materials.

N. Kröger, K. H. Sandhage

MRS Bull. 35, 122-126 (2010).

Characterization of an endoplasmic reticulum-associated silaffin kinase from the diatom Thalassiosira pseudonana

V. C. Sheppard, N. Poulsen, N. Kröger

J. Biol. Chem. 285, 1166-1176 (2010).


Protein-Mediated Layer-by-Layer Syntheses of Freestanding Microscale Titania Structures with Biologically Assembled 3-D Morphologies.

Y. Fang, Q. Wu, M. B. Dickerson, Y. Cai, S. Shian, J. Berrigan, N. Poulsen, N. Kröger, K. H. Sandhage

Chem. Mater. 21, 5704-5710 (2009).

The molecular basis of nacre formation

N. Kröger

Science 325, 1351-1352 (2009).

Protein-Enabled Synthesis of Monodisperse Titania Nanoparticles On and Within Polyelectrolyte Matrices.

E. Kharlampieva, J. M. Slocik, S. Singamaneni, N. Poulsen, N. Kröger, R. R. Naik, V. V. Tsukruk

Adv. Funct. Mat. 19: 2303-2311 (2009).


Diatoms - from cell wall biogenesis to nanotechnology

N. Kröger, N. Poulsen

Annu. Rev. Genet. 42, 83-107 (2008).

The Phaeodactylum genome reveals the evolutionary history of diatom genomes.

C. Bowler, et al.

Nature. 456, 239-244 (2008).

Bio-enabled Surface-mediated Growth of Uniform Titania Nanoparticles

E. Kharlampieva, T. Tsukruk, J. M. Slocik, H. Ko, N. Poulsen, R. R. Naik, N. Kröger, V. V. Tsukruk

Adv. Mater. 20, 3274-3279 (2008).

Identification of peptides capable of inducing the formation of titania but not silica via a subtractive bacteriophage display approach

Y. Fang, N. Poulsen, M. B. Dickerson, Y. Cai, S. E. Jones, R. R. Naik, N. Kröger, K. H. Sandhage

J. Mater. Chem. 18, 3871-3875 (2008).


Prescribing diatom morphology: toward genetic engineering of biological nanomaterials

N. Kröger

Cur. Opin. Chem. Biol. 11, 662-669 (2007).

Biochemistry and Molecular Genetics of Silica Biomineralization in Diatoms

N. Kröger, N. Poulsen

Handbook of Biomineralization - Biological Aspects and Structure Formation (E. Baeuerlein, ed.), Wiley-VCH, p. 43-58 (2007)

Silica immobilization of an enzyme via genetic engineering of the diatom Thalassiosira pseudonana

N. Poulsen, C. Berne, J. Spain, N. Kröger

Angew. Chem. Int. Ed. 46, 1843-1846 (2007).

Identification and Design of Peptides for the Rapid, High Yield Formation of Nanoparticulate TiO2 from Aqueous Solutions at Room Temperature

M. B. Dickerson, S. E. Jones, Y. Cai, G. Ahmad, R. R. Naik, N. Kröger, K. H. Sandhage

Chem. Mater. 20, 1578-1584 (2007).


Bio-enabled synthesis of Rutile (TiO2) at ambient temperature and neutral pH

N. Kröger, M. B. Dickerson, G. Ahmad, Y. Cai, M. S. Haluska, K. H. Sandhage, N. Poulsen, V. C. Sheppard

Angew. Chem. Int. Ed. 45, 7239-7243 (2006).

Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae)

N. Poulsen, P. M. Chesley, N. Kröger

J. Phycol. 42, 1059-1065 (2006).

Rapid, Room-Temperature Formation of Crystalline Calcium Molybdate Phosphor Microparticles via Peptide-Induced Precipitation

G. Ahmad, M. B. Dickerson, C. Church, Y. Cai, S. E. Jones, R. R. Naik, J. S. King, J. C. Summers, N. Kröger, K. H. Sandhage

Adv. Mater. 18, 1759-1763 (2006).


Biosilica Nanofabrication in Diatoms: The Structures and Properties of Regulatory Silaffins.

N. Kröger, N. Poulsen

MRS Proceedings, 873, K1.2 doi:10.1557/PROC-873-K1.2 (2005)

A new molecular tool for transgenic diatoms: Control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette

N. Poulsen, N. Kröger

FEBS J. 272, 3413-3423 (2005).


Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana

N. Poulsen, N. Kröger

J. Biol. Chem. 279, 42993-42999 (2004)

The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism

E. V. Ambrust, et al.

Science 306, 79-86 (2004).

The Molecular Basis of Diatom Biosilica Formation

N. Kröger, M. Sumper

in Biomineralization (E. Baeuerlein, ed.) 2nd edition, Wiley-VCH, p. 137-158 (2004)

Silica formation in diatoms: the function of long-chain polyamines and silaffins

M. Sumper, N. Kröger

J. Mater. Chem. 14, 2059-2065 (2004).


Biosilica formation in diatoms: Characterization of native silaffin-2 and its role in silica morphogenesis

N. Poulsen, M. Sumper, N. Kröger

Proc. Natl. Acad. Sci. USA 100, 12075-12080 (2003).

Solid-state 29Si MAS NMR studies of diatoms: structural characterization of biosilica deposits

R. Bertermann, N. Kröger, R. Tacke

Anal. Bioanal. Chem. 375, 630-634 (2003).

Silica deposition by a repetitive proline-rich protein from systemically resistant cucumber plants

H. Kauss, K. Seehaus, R. Franke, S. Gilbert, R. Dietrich, N. Kröger

Plant J. 33, 87-95 (2003).


Self-assembly of highly phosphorylated silaffins and their function in biosilica morphogenesis

N. Kröger, S. Lorenz, E. Brunner, M. Sumper

Science 298, 584-586 (2002).


The sweetness of diatom molecular engineering

N. Kröger

J. Phycol. 37, 657-658 (2001).

Silica precipitating peptides from diatoms: The chemical structure of silaffin-1A from Cylindrotheca fusiformis

N. Kröger, R. Deutzmann, M. Sumper

J. Biol. Chem. 276, 26066-26070 (2001).

1H, 13C and 15N sequence-specific resonance assignment of the PSCD4 domain of diatom cell wall protein pleuralin-1

M. Wenzler, E. Brunner, N. Kröger, G. Lehmann, M. Sumper, H. R. Kalbitzer

J. Biomolec. NMR 20, 191-192 (2001).

The biogenesis of silicate cell walls of diatoms

N. Kröger

BIOspektrum 7, 556-557 (2001).


Species specific polyamines from diatoms control silica morphology

N. Kröger, R. Deutzmann, C. Bergsdorf, M. Sumper

Proc. Natl. Acad. Sci. USA 97, 14133-14138 (2000).

Pleuralins are involved in theca differentiation in the diatom Cylindrotheca fusiformis

N. Kröger, R. Wetherbee

Protist 151, 263-273 (2000).

The Biochemistry of Silica Formation in Diatoms

N. Kröger, M. Sumper

in Biomineralization (E. Baeuerlein, ed.) 1st edition, Wiley-VCH, p. 151-170 (2000)


Polycationic peptides from diatom biosilica that direct silica nanosphere formation

N. Kröger, R. Deutzmann, M. Sumper

Science 286, 1129-1132 (1999).

Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae)

H. Fischer, I. Robl, M. Sumper, N. Kröger

J. Phycol. 35, 113-120 (1999).


Diatom cell wall proteins and the cell biology of silica biomineralization

N. Kröger and M. Sumper

Protist 149, 213-219 (1998)


Characterization of a 200 kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall

N. Kröger, G. Lehmann, R. Rachel, M. Sumper

Eur. J. Biochem. 250, 99-105 (1997)


Frustulins: domain conservation in a protein family associated with diatom cell walls

N. Kröger, C. Bergsdorf, M. Sumper

Eur. J. Biochem. 239, 259-264 (1996)


A new calcium binding glycoprotein family constitutes a major diatom cell wall component

N. Kröger, C. Bergsdorf, M. Sumper

EMBO J. 13, 4676-4683 (1994)