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Publication: Programmable Patterning of Protein Bioactivity by Visible Light

NanoLetters, in press (2014)

The simple and quick patterning of functional proteins on engineered surfaces affords an opportunity to fabricate protein microarrays in lab-on-chip systems. In this publication, a novel approach towards the the programmable patterning of proteins as well as the local activation of enzymes by visible light is reported. Specifically, the light locally heats the surface and switches the conformation of a thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) polymer coating which the proteins are adsorbed into. As the conformation of the polymers determines the binding and the activity kinestics of the proteins, the specific patterning of multiple kinds of proteins side-by-side by sequential processing without the need for specific linker molecules or elaborate surface preparations could be demonstrated. Advantages of using visible light (instead of ultraviolet or infrared light) in the proposed photothermal patterning approach are that it is (i) inoffensive to proteins, (ii) versatile regarding the wavelength range, and (iii) easy to implement into a conventional fluorescence microscope.

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C. Reuther, R. Tucker, L. Ionov, S. Diez: Programmable patterning of protein bioactivity by visible light. (publication date web: Jun 9, 2014)

DOI: 10.1021/nl501521q

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