Twist and torque in the cellular nanocosmos: Diez group detects biophysical forces of molecular motors in 3D
Publication: Simultaneous single-molecule force and fluorescence sampling of DNA nanostructure conformations using magnetic tweezers
In this publication, the team of Michael Schlierf and co-workers present a hybrid single-molecule technique combining magnetic tweezers and Förster resonance energy transfer (FRET) measurements. Through applying external forces to a paramagnetic sphere, they induce conformational changes in DNA nanostructures, which are detected in two output channels simultaneously. First, by tracking a magnetic bead with high spatial and temporal resolution, they observe overall DNA length changes along the force axis. Secondly, the measured FRET efficiency between two fluorescent probes monitors local conformational changes. The synchronized orthogonal readout in different observation channels will facilitate deciphering the complex mechanisms of biomolecular machines.
Felix E Kemmerich, Marko Swoboda, Dominik J Kauert, Maj Svea Grieb, Steffen Hahn, Friedrich W Schwarz, Ralf Seidel, and Michael Schlierf: Simultaneous single-molecule force and fluorescence sampling of DNA nanostructure conformations using magnetic tweezers, DOI: 10.1021/acs.nanolett.5b03956
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