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Publication: Structural stability of DNA origami nanostructures in the presence of chaotropic agents
DNA origami represent powerful platforms for single-molecule investigations of biomolecular processes. The required structural integrity of the DNA origami may, however, pose significant limitations regarding their applicability, for instance in protein folding studies that require strongly denaturing conditions. Here, we therefore report a detailed study on the stability of 2D DNA origami triangles in the presence of the strong chaotropic denaturing agents urea and guanidinium chloride (GdmCl) in dependence of concentration and temperature. At room temperature, the DNA origami triangles are stable up to at least 24 h in both denaturants at concentrations as high as 6 M. At elevated temperatures, however, structural stability is governed by variations in melting temperature of the individual staple strands. Therefore, the global melting temperature of the DNA origami does not represent an accurate measure of their structural stability. Overall, their high stability renders DNA origami promising platforms for biomolecular studies in the presence of chaotropic agents, including single-molecule protein folding or structural switching.
Structural stability of DNA origami nanostructures in the presence of chaotropic agents
Saminathan Ramakrishnan, Georg Krainer, Guido Grundmeier, Michael Schlierf and Adrian Keller
Nanoscale, 2016 DOI: 10.1039/C6NR00835F
First published online 21 Apr 2016
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