Schlierf Group - Protein-DNA interactions
DNA replication initiation
Initiation of DNA replication is an essential step in cell proliferation of all organisms. Defects in the start of replication can be correlated to DNA damage as well as genetic instability. DNA replication is initiated across all domains of life by so-called initiator proteins interacting with replication origin sites and coordinating the replisome assembly. We are interested in the origin melting mechanism in bacterial and bacteriophage proteins as model systems. We use the single-molecule FRET and single-molecule PIFE to observe the dynamic process of DNA binding events at the molecular level.
Team members on the project: Hsin-Mei, Philip, Andreas
- Cheng, Gröger, Hartmann, Schlierf (2015) Bacterial initiators form dynamic filaments on single-stranded DNA monomer by monomer, Nucleic Acids Research
The Bacterial Integron
The bacterial DNA recombination system called integron is predominantly involved in the spread of antibiotic resistances. Integron recombination is unique as its recombinase, IntI, can target a single-stranded recombination site, called attC. attC sites fold themselves to form an imperfect hairpin structure of 65 to 150 nucleotides, resembling a canonical recombination site. Though the integron plays an important role in human pathology, it is unknown how the attC hairpins form in the bacterial cytoplasm and how they are recognised and stable in the presence of numerous competing factors. We use single-molecule FRET and FCS to study the interaction of IntI and SSB with the attC site aadA7.
Team members on the project: Svea, Varsha